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Viral RNA was precipitated from 100 μl of Vs virus-infected mouse brain ... and 3 μl of deoxynucleoside triphosphates (10 mM), 3 μl of dithiothreitol (0.1 mM), 1 μl of ... text, were taken from the literature (8, 14, 15, 21, 22, 28, 30, 34, 47, 49, 86, 87). .... For each virus, there was a direct correlation between the initial MOI and the .... Isolation and Characterization of Bacteria from Ancient Siberian Permafrost Sediment ... Direct microbial counts after natural permafrost enrichment showed the ... °C or 30 °C. Sensitivity to rich media, antibiotics, heavy metals, and salt increased ..... ago [34], and probably earlier than 3.5 million years ago, and never thawed .... assay of nuclear staining with crystal violet after 30-180 mm co-culture. ...... 34,7 ns. 35,0 ns. 21,4 ns. 47,3 ns. 46,4. *150. TAU. C. 121,2. 160,8. 160,5. 179,9. 126,7 ... collecting ducts, suggesting a direct action of acetyicholine on renal tubule cells. ...... 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